추천 논문

Next-Generation Sequencing Technology: Current Trends and Advancements

https://www.mdpi.com/2079-7737/12/7/997

first_pagesettingsOrder Article Reprints

Open AccessReview

Next-Generation Sequencing Technology: Current Trends and Advancements

by Heena Satam 1,Kandarp Joshi 1ORCID,Upasana Mangrolia 1ORCID,Sanober Waghoo 1,Gulnaz Zaidi 1ORCID,Shravani Rawool 1,Ritesh P. Thakare 2ORCID,Shahid Banday 2,Alok K. Mishra 2ORCID,Gautam Das 1,* andSunil K. Malonia 2,*ORCID

1

miBiome Therapeutics, Mumbai 400102, India

2

Department of Molecular Cell and Cancer Biology, UMass Chan Medical School, Worcester, MA 01605, USA

*

Authors to whom correspondence should be addressed.

Biology 2023, 12(7), 997; https://doi.org/10.3390/biology12070997

Received: 22 June 2023 / Revised: 9 July 2023 / Accepted: 11 July 2023 / Published: 13 July 2023

(This article belongs to the Section Biotechnology)

Download Browse Figures Review Reports Versions Notes

Simple Summary

Next-generation sequencing (NGS) is a powerful tool used in genomics research. NGS can sequence millions of DNA fragments at once, providing detailed information about the structure of genomes, genetic variations, gene activity, and changes in gene behavior. Recent advancements have focused on faster and more accurate sequencing, reduced costs, and improved data analysis. These advancements hold great promise for unlocking new insights into genomics and improving our understanding of diseases and personalized healthcare. This review article provides an overview of NGS technology and its impact on various areas of research, such as clinical genomics, cancer, infectious diseases, and the study of the microbiome.

Abstract

The advent of next-generation sequencing (NGS) has brought about a paradigm shift in genomics research, offering unparalleled capabilities for analyzing DNA and RNA molecules in a high-throughput and cost-effective manner. This transformative technology has swiftly propelled genomics advancements across diverse domains. NGS allows for the rapid sequencing of millions of DNA fragments simultaneously, providing comprehensive insights into genome structure, genetic variations, gene expression profiles, and epigenetic modifications. The versatility of NGS platforms has expanded the scope of genomics research, facilitating studies on rare genetic diseases, cancer genomics, microbiome analysis, infectious diseases, and population genetics. Moreover, NGS has enabled the development of targeted therapies, precision medicine approaches, and improved diagnostic methods. This review provides an insightful overview of the current trends and recent advancements in NGS technology, highlighting its potential impact on diverse areas of genomic research. Moreover, the review delves into the challenges encountered and future directions of NGS technology, including endeavors to enhance the accuracy and sensitivity of sequencing data, the development of novel algorithms for data analysis, and the pursuit of more efficient, scalable, and cost-effective solutions that lie ahead.

Keywords: next-generation sequencing; genomics; microbiome; molecular diagnostics; bioinformatics; Nanopore; PacBio; Illumina; pyrosequencing