추천 논문

Methods for the detection and identification of pathogenic bacteria: past, present, and future

https://pubs.rsc.org/en/content/articlehtml/2017/cs/c6cs00693k

Abstract

In order to retard the rate of development of antibacterial resistance, the causative agent must be identified as rapidly as possible, so that directed patient treatment and/or contact precautions can be initiated. This review highlights the challenges associated with the detection and identification of pathogenic bacteria, by providing an introduction to the techniques currently used, as well as newer techniques that are in development. Focusing on the chemical basis for these techniques, the review also provides a comparison of their advantages and disadvantages.

Key learning points

(1) Why rapid identification of pathogenic bacterial is necessary.

(2) The requirements of a bacterial detection and identification method.

(3) A comparison of the attributes of culture-based and molecular methods for the detection of bacteria.

(4) The chemical basis of the techniques used in the identification of pathogenic bacteria.

(5) Chromogenic and fluorogenic enzyme substrates for bacterial detection.